Wednesday, January 11, 2012

Wrap up of tweets from Joe Derisi talk

Just a quick post here - for those not following on Twitter - Joe Derisi gave a talk at UC Davis 1/9 and I posted some tweets about it.  Here they are:

phylogenomics
Joe DeRisi getting ready for his talk at the #ucdavis Genome Center this am http://t.co/OwZbM2nn
1/9/12 9:57 AM


phylogenomics
Joe Derisi's first slide : "Bees, viruses, and plastids: a seminar in two parts" - I think he needs to work on his math http://t.co/0nPjChlc
1/9/12 10:05 AM





phylogenomics
DeRisi works with mobile honeybee colony trucks that travel around the country to service pollination needs
1/9/12 10:09 AM



phylogenomics
DeRisi - one of the problems with figuring out what is causing CCD is we do not know much about "normal" healthy colonies
1/9/12 10:09 AM



phylogenomics
Bee trucks start in Mississippi in winter, spring in Dakotas, then To california for almonds
1/9/12 10:10 AM


phylogenomics
DeRisi got into bee studies because of colony collapse disorder CCD
1/9/12 10:13 AM



phylogenomics
DeRisi - grinds up bees and then assays them with "bee pathogen chip" and sequencing
1/9/12 10:13 AM



phylogenomics
Derisi discussing his new Plos one w/ SFSU paper showing phorid flies associated with bees
1/9/12 10:16 AM



phylogenomics
Derisi says if you want your paper to get 1000s of hits you should mention Zombies somewhere as they did w/ "Zombie bees"
1/9/12 10:18 AM



phylogenomics
Derisi: zombie bees are cool but the phorids are probably not associated with CCD
1/9/12 10:19 AM



phylogenomics
DeRisi found six major pathogens associated w/ bees: Nosema, phorids, spiroplasma, notovirus, and others
1/9/12 10:22 AM



phylogenomics
DeRisi - sequencing ground up bees - most reads are host or pathogens they know about - says key to characterizing new viruses is assembly
1/9/12 10:23 AM



phylogenomics
DeRisi mentioning their PRICE assembler - an inductive assembly method
1/9/12 10:25 AM




notSoJunkDNA
@phylogenomics URL for PRICE is http://t.co/bRGiY7Rz
1/9/12 10:30 AM



phylogenomics
Why I love Joe Derisi "there will be a manuscript on the assembler someday... But the software is available for free now"
1/9/12 10:27 AM




phylogenomics
DeRisi pooled together Bee samples prepped with various methods so that he could get diverse viruses covered
1/9/12 10:29 AM




phylogenomics
DeRisi - lake sinai virus 2 is present in up to 10^11 copies per bee
1/9/12 10:35 AM



phylogenomics
DeRisi - also present in bees - Crithidia - a trypanosome - related to known pathogens of other insects
1/9/12 10:36 AM



phylogenomics
DeRisi - Crithidia also increases in abundance in winter
1/9/12 10:36 AM




phylogenomics
DeRisi - sequenced and assembled genome of Crithidia - genome is interesting
1/9/12 10:37 AM




phylogenomics
DeRisi - part two - the essential function of the Plasmodium apicoplast
1/9/12 10:38 AM



phylogenomics
DeRisi - history of Studies of plasmodium apicoplast - organelle bound by four membranes - likely b/c result of secondary symbiosis
1/9/12 10:39 AM



phylogenomics
Derisi discussing sequencing of apicoplast genome in 1990s by Gardner, McFadden, etc
1/9/12 10:40 AM



phylogenomics
DeRisi - experiments suggest IPP is the sole essential product of apicoplast biology
1/9/12 10:47 AM








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