Thursday, October 18, 2012

Important read: Biotechniques discussion of DNA extraction in microbial studies

Very quick post here.  This is worth a read: BioTechniques - DNA Extraction: Overcoming Obstacles in Microbial Studies.  From their summary:

"What are the most efficient methods to extract microbial DNA that accurately represents the community it is isolated from? Janelle Weaver reports on efforts to identify the best methods for DNA extraction from unknown frontiers in the human body and across the globe."

It discusses among many things this fascinating paper: Flores GE, Henley JB, Fierer N (2012) A Direct PCR Approach to Accelerate Analyses of Human-Associated Microbial Communities. PLoS ONE 7(9): e44563. doi:10.1371/journal.pone.0044563

Note - I found out about this on the Twitter

On a related note see the paper from my lab on a metagenomic simulation we did a few years ago ...

PLOS ONE: Metagenomic Sequencing of an In Vitro-Simulated ...

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