Tuesday, April 08, 2008

PLoS Biology - Darwinian Evolution on a Chip

For evolution afficionados, there is a cool new paper in PLoS Biology on using a microfluidic chip to conduct in vitro evolution experiments.

The paper, by Brian Paegel and Gerry Joyce from the Scripps Research Institute
relies on computer control and microfluidic chip technology to automate the directed evolution of functional molecules, subject to precisely defined parameters. We used a population of billions of RNA enzymes with RNA-joining activity, which were challenged to react in the presence of progressively lower concentrations of substrate. The enzymes that did react were amplified to produce progeny, which were challenged similarly. Whenever the population size reached a predetermined threshold, chip-based operations were executed to isolate a fraction of the population and mix it with fresh reagents. These steps were repeated automatically for 500 iterations of 10-fold exponential growth followed by 10-fold dilution.
They have a nice figure summarizing the system which I show below --- and people should check out the article. Note - I can legally include this figure here because of the use of a broad Creative Commons License by PLoS Biology. Note - one of the stories I saw about this at Medgadget.Com also pointed out how they can use the article however they want because of where it was published. The power of true Open Access.



(A) The evolution chip is mounted on a temperature-controlled stage. Solutions containing polymerase enzymes (E) and mono- and oligonucleotide components (S) are delivered to the chip via capillary tubing and output to a pressure-controlled collection vial (O). A microscope objective is used to focus laser excitation (λex = 490 nm) on the microfluidic channel and to gather fluorescence (λem = 535 nm), which is detected with a confocal PMT. Valve actuation and fluid flow are controlled by six independent vacuum lines.

(B) The microfluidic device is shown with the active circuit filled with blue dye.

(C) The serial dilution circuit consists of a mixing loop with fluid flow channels (red), fluid access reservoirs (blue), and control valves (black). Fluid flow around the loop is controlled by three two-way valves (a, b, and c). Fluid access to the loop from the input reservoirs (RE and RS) and to the output reservoir (RO) is controlled by bus valves (in and out). The bus valves allow access when open, and prevent access while preserving fluidic continuity within the loop when closed.

(D) During operation of the circuit, the expanding RNA population is incubated while undergoing slow cyclic mixing until the fluorescence reaches a pre-determined threshold. Then an aliquot of the population is isolated between valves in and out as fresh solutions of E and Sin and out, and the aliquot is mixed with the fresh solutions by rapid serial actuation of valves a, b, and c. Open valves are indicated by filled circles; closed valves are indicated by a red X. are drawn into the loop and spent materials are delivered to the collection vial. Finally, the loop is sealed by closing valves

From: Darwinian Evolution on a Chip Paegel BM, Joyce GF PLoS Biology Vol. 6, No. 4, e85 doi:10.1371/journal.pbio.0060085


NIH Mandate on Open Access - Good First Step, Thanks to PLoS et al, but still a long way to go

Well, today is the day. The day after the new NIH mandate on Open Access (also see here for more information) to publications has begun. I think this is a great great day for science. And for society and Congress should be commended for doing something that is good for the country and the world that may have upset some of their big donors (i.e., the publishing industry).

And I think we all owe a big round of thanks to those who worked towards this goal. Clearly, there were many involved in convincing Congress to do this, from concerned members of the public, to SPARC and other NGOs, to tireless individuals like Peter Suber, and of course the Public Library of Science. For those who do not know, or may have forgotten, the Public Library of Science got it's beginnings as an initiative to promote Open Access publishing (and it was not initially a publisher of journals). PLoS was founded by Harold Varmus, Pat Brown and Michael Eisen (my brother) in 2000. The first thing they did was circulate a petition to promote open access publishing. I signed the letter as did many many others. But alas, it was not enough. And so PLoS started its journals and many realized that it would be necessary for funding agencies to step in and require Open Access publishing for work they funded. And after a long struggle, we are now here with the new NIH mandate (as well as mandates from other agencies which got there before NIH). And I think that we all owe a big thanks to everyone behind this initiative. Also it is worth checking out Harold Varmus' essay today on the new NIH initiative in PLoS Biology.

I note, however, that the NIH policy is only a first step. It does not move us completely towards true Open Access to scientific publications. There are still issues that need to be addressed, including the timing of release of publications (I think everything should be released immediately, not after a 6 or 12 month delay), the issue of Copyright, the need to get old publications into the public domain, and how putting material in Pubmed Central does not completely open it up to the world (see Peter Murray-Rust's very interesting discussion of this on his blog). So there is still much work to be done. But nevertheless, I am happy to be living in this new world where NIH has made OA a key part of it's mandate.

Monday, April 07, 2008

Congrats to Amy Harmon: Pulitzer for DNA Age Stories.

Congrats to Amy Harmon, reporter for the New York Times, who writes a lot about DNA (see all the DNA Age stories), who won one of those Pulitzer things today. I guess, maybe leaving me out of one of your last stories was a good call. I have written about her stories before a few times and overall, I like her reporting. What we all really need to do is convince her to start a blog. Print media is so 21st century.

Also see some other stories:

Passing of Jeremy Knowles

I write with sadness that Jeremy Knowles has passed away. Knowles was a Professor at Harvard, had been Dean of the Faculty of Arts and Sciences at Harvard, and an HHMI Trustee, among many things. I did not know him well, but I did have a recent scientific exchange with him regarding analysis we were doing of the Tetrahymena genome. Knowles has asked a question to Peter Bruns, who is a Tetrahymena guru (and also was at HHMI). The question related to intron splice junctions and whether that might support a paper he had written in 1992. The science here is not important.

I wrote back, basically saying we were sequencing the genome but alas our data might not be useful for his question since most of what we were doing at the time was predicting gene splice sites not actually determining them for real. And I said, in fact, much of the data in Genbank was also predictions not real cDNAs.

I expected him to be a bit disappointed. But instead, he wrote back to Peter:
Peter:
Thank you! Jonathan's response confirms that I was right to ask an expert. For if I had gone fishing in the gene bank pool, I should probably have drowned. I shall wait, calmly.
best,
Jeremy
What I was struck with was his sense of humor and warmth, which emanated from this and a few other simple email messages. Based on these communications, I was looking forward to interacting with him again as we are now writing up a paper including all of our new cDNA data (real sequences, not predictions). I am sorry to see him go.

Sunday, April 06, 2008

Tree of Life Art

As someone who studies the "Tree of Life" in terms of evolution (the tree of life is an evolutionary tree relating all life forms) and who even named his blog after this, I am fascinated by different portrayals of the Tree of Life. We can see lots of things like the Tree of Life in the real word. Much of this is due to the use of the Tree of Life imagery by various religious groups. Some of it is more connected to evolution in some way. But whatever the inspiration, there are some pretty nice representations out there. And I am posting one of them today. At UC Davis the UC Davis Arboretum is a really spectacular place. I go walking there all the time and take my kids to play there and watch the ducks and other animals. One day, I was walking near some gardens that they have been renovating for a while, when I saw they had unveiled some new artwork.

Below are some pictures of this artwork.  I am going to start posting more "Tree of Life" art here in the future and would love to get examples from people out there too.

More on Tree of Life art in Arboretum

As someone who studied "The tree of Life" in terms of evolution I am fascinated by the Tree of Life art in the Arboretum.  Here are some more pictures




Friday, April 04, 2008

Can a spoof be scooped by reality?

Well, if you read this blog, or a few others, you may already know that what I reported on here on April 1 regarding NIH cracking down on brain doping, was, in fact an April Fool's joke. I will write more about this joke later and how we planned it, starting at something called scifoo camp at Google headquarters in the summer of last year. But I do want to write about one thing here -- we sort of got scooped by reality. I had been planning for some time to do an April 1 joke on performance enhancing drug use among scientists. And as I was recruiting others to be involved in this plot, reality got in the way when Nature and then the New York Times started to report on brain doping as a real issue. It kind of took the absurdity away from our joke and made it seem like we were making some real commentary on brain doping when in fact, we were at least originally just trying to come up with something ridiculous but possibly believable at first look.

And now it has happened again. The second spoof I considered doing was to write about doing DNA and drug testing on some water bottles I collected from the Tour of California - a bike race that came 1 block from my house. I went for a little ride on the route of the race a few minutes after the peloton came by and got about 10 bottles from different teams. (This was real). And then I was going to pretend that we took those bottles and (1) figured out whose they were by DNA testing and (2) did drug tests of them. And this would allow me to write about 23 and me and other DNA testing companies as well as to make some fun of performance enhancing drug scandals. And I thought this would be ridiculous in a way because it could not possibly be legal to test someone's DNA without any real just cause. And low and behold look at what comes along --- Amy Harmon has an article in the Times yesterday on people doing exactly this type of thing --- testing other people's DNA (good article, by the way).

So now twice my spoofs end up getting scooped by reality. To me, a good spoof/April 1 joke is completely absurd in hindsight even if it is believable initially. So every time one of these jokes/pranks I want to pull gets close to reality, it becomes much much less funny to me. Who knows, maybe Craig Venter will in fact sequence his own microbiome, as I joked about last April 1.

Maybe before my next joke, I should call up Amy Harmon and other reporters to make sure they are not working on related stories first?

Open Evolution: Google supports open source code for evolutionary studies - Deadline 4-08 for Summer of Code

See email I received via Iddo Friedberg from the National Evolutionary Synthesis Center which is seeking students and others to participate in the Phyloinformatics Summer of Code.

This is just a reminder that the student application period ends
definitively on April 7 (Monday), 5pm PDT/8pm EDT (0:00 UTC on April
8). (Google extended the period from the original end date of March
31.) There are still a few project ideas that have received little
attention from student applications so far. If you are interested,
please see below for URLs, and get in touch with us phylosoc {at}
nescent {dot} org to inquire for more information re: application
status of your favorite project(s).

Phyloinformatics Summer of Code 2008
http://phyloinformatics.net/Phyloinformatics_Summer_of_Code_2008

*** Please disseminate this announcement widely to appropriate students
at your institution ***

The National Evolutionary Synthesis Center (NESCent: http://
www.nescent.org/
) is participating in 2008 for the second year as a
mentoring organization in the Google Summer of Code (http://
code.google.com/soc
). Through this program, Google provides
undergraduate, masters, and PhD students with a unique opportunity to
obtain hands-on experience writing and extending open-source software
under the mentorship of experienced developers from around the world.

Our goal in participating is to train future researchers and
developers to not only have awareness and understanding of the value
of open-source and collaboratively developed software, but also to
gain the programming and remote collaboration skills needed to
successfully contribute to such projects. Students will receive a
stipend from Google, and may work from their home, or home
institution, for the duration of the 3 month program. Students will
each have one or more dedicated mentors with expertise in
phylogenetic methods and open-source software development.

NESCent is particularly targeting students interested in both
evolutionary biology and software development. Project ideas (see URL
below) range from visualizing phylogenetic data in R, to development
of a Mesquite module, web-services for phylogenetic data providers or
geophylogeny mashups, implementing phyloXML support, navigating
databases of networks, topology queries for PhyloCode registries, to
phylogenetic tree mining in a MapReduce framework, and more.

The project ideas are flexible and many can be adjusted in scope to
match the skills of the student. If the program sounds interesting to
you but you are unsure whether you have the necessary skills, please
email the mentors at the address below. We will work with you to
find a project that fits your interests and skills.

INQUIRIES:
Email any questions, including self-proposed project ideas, to
phylosoc {at} nescent {dot} org.

TO APPLY:
Apply on-line at the Google Summer of Code website
(http://code.google.com/soc/2008), where you will also find GSoC program
rules and eligibility requirements. The 1-week application period for
students opened on Monday March 24th and runs through Monday, March
31st, 2008.

Hilmar Lapp and Todd Vision
US National Evolutionary Synthesis Center

=====
URLs:
=====

2008 NESCent Phyloinformatics Summer of Code:
http://phyloinformatics.net/Phyloinformatics_Summer_of_Code_2008

Eligibility requirements:
http://code.google.com/opensource/gsoc/2008/faqs.html#0.1_eligibility

Stipends:
http://code.google.com/opensource/gsoc/2008/faqs.html#0.1_administrivia

Quote of the week "There are much yummier and easier things to eat in the human body"

An interesting new paper has appeared in one of the those non Open Access journals - Science. Normally I do not write about such closed access, closed minded things, but I want to make an exception here. The paper is by George Church and colleagues on bacteria that subsist on a diet of antibiotics.

The paper is perfectly reasonable, if not pretty cool. Although it is a bit over hyped. It is obvious that bacteria would be able to eat antibiotics. Not only has this been shown previously, (as Church is very careful to point out) but bacteria eat anything and everything. So of course they will eat energy rich antibiotic molecules. The real problem in this case is the fear mongering that will come with the reporting on this. In the first few articles we already see this. For example, Reuters says
Church said the finding underscores the extent to which bacteria have developed resistance to antibiotics, a process that started almost as soon as penicillin was introduced in the 1940s.
Ummm ... antibiotics have actually been around for billions of years. Microbes make them all the time. So resistance too has been around for a long time. Yes, we are selecting for strains resistant to all our classes of antibiotics. But resistance itself did not start with the introduction of penicillin. I note the AP story got this right:
Nor is it a surprise that soil bacteria can withstand some antibiotics; some had already been found. After all, a number of antibiotics are natural — think penicillin. Some antibiotics have been derived from soil.
The only sense in all the stories I saw came from Jo Handelsman who was quoted in many of them:
"Thank goodness we have those bacteria to eat at least some of the antibiotics," said bacteriologist Jo Handelsman of the University of Wisconsin-Madison, who wasn't involved in the study. "Nature's pretty effective." From AP article.
Even better, Handelsman tried to calm the fears about whether bacteria would eat antibiotics given to treat infections:
But bacteriologist Jo Handelsman of the University of Wisconsin, Madison thinks this is unlikely, as “there are much yummier and easier things to eat in the human body."
And for that, she gets the quote of the week.

Wednesday, April 02, 2008

Bill and Melinda (Gates that is) ask for your ideas ... lots of money on the line

Well, I signed up for emails from the Bill and Melinda Gates Foundation, hoping to apply for some of their trillions of dollars to do a little bit of research.

And I got an email today I would like to share, since, well, they said I should share it. And here it is ..
The Bill & Melinda Gates Foundation is now accepting grant proposals for Grand Challenges Explorations, a US$100 million initiative to help scientists pursue innovative ideas for solving major global health problems.

Grant proposals are being accepted online at www.gcgh.org/explorations until May 30, 2008, on the following topics:

-- Creating new ways to protect against infectious diseases
-- Creating drugs or delivery systems that limit the emergence of resistance
-- Creating new ways to prevent or cure HIV infection
-- Exploring the basis for latency in TB

Initial grants will be $100,000 each, and projects showing success will have the opportunity to receive additional funding of $1 million or more. Full descriptions of the topics and application instructions are available at www.gcgh.org/explorations.

We are looking forward to receiving innovative ideas from scientists around the world and from all scientific disciplines. If you don't submit a proposal yourself, we hope you will forward this message to someone else who might be interested.

Thank you for your commitment to solving the world's greatest health challenges.

###
Bill & Melinda Gates Foundation

Guided by the belief that every life has equal value, the Bill & Melinda Gates Foundation works to reduce inequities and improve lives around the world. In developing countries, it focuses on improving health, reducing extreme poverty, and increasing access to technology in public libraries. In the United States, the foundation seeks to ensure that all people have access to a great education and to technology in public libraries. In its local region, it focuses on improving the lives of low-income families. Based in Seattle, the foundation is led by CEO Patty Stonesifer and Co-chairs William H. Gates Sr., Bill Gates, and Melinda French Gates.

Tuesday, April 01, 2008

Davis Life Magazine

Just got an email announcing a new issue of Davis Life Magazine which I meant to post about here previously. It is a nice little web magazine about, well, life in Davis.

What is so bad about brain doping? Apparently, NIH thinks something is.

UPDATE - FAKE SCIENCE NEWS HERE.

Recently, there has been a lot of talk around the web about brain doping ... that is, using some sort of drug to enhance intelligence or intellectual performance in some way. For example see

A key question is - is brain doping bad? I am not so sure. However, NIH apparently thinks otherwise. NIH has just announced the formation of the World Anti Brain Doping Authority in conjunction with the ever so annoying World Anti Doping Authority. For more information see http://wabda.org and I attach the announcement below. Apparently, they are working on making a list of banned drugs and punishment for misuse for anyone funded by NIH. I have also heard they will be doing drug testing to new grant awardees. Lovely.

What is funny is this so fits in with the new top down style at the NIH. Forget about the individual scientist and the need for independence and creativity. There are so many rules about everything now that I think I will just seek out funds from other agencies.

See also other bloggers writing about this:
Here is the announcement:

NIH Announces New Initiatives to Fight the Use of Brain Enhancing Drugs by Scientists

The National Institutes of Health (NIH) today announced three new initiatives to fight the use of brain enhancing drugs by scientists. The new initiatives are (1) the creation of the NIH Anti-Brain Doping Advisory Group (NABDAG), a new trans-NIH committee, (2) a collaboration with the World Anti-Doping Authority (WADA) and the European Commission to create the World Anti-Brain Doping Authority (WABDA) and (3) the adoption by the NIH of the World Anti-Brain Doping Code – a set of regulations on the use of brain enhancing drugs among scientists.

"These new initiatives are designed to level the playing field among scientist in terms of intellectual activities," said NIH Director Elias A. Zerhouni, M.D. "These three activities are designed to get NIH ahead of the curve in terms of performance enhancing drug use among scientists."

NABDAG will serve to coordinate activities across different NIH agencies in terms of regulating the use of brain enhancing drugs. The trans-NIH group will be directed by internationally renowned doping authority Jonathan Davis, Ph.D., current director of research at WADA.

"The priority of NABDAG will be to seek out input from the scientific community and from within NIH," Davis said. "The availability of tremendous expertise and the remarkable infrastructure at NIH will make our activities more robust and will allow us to tackle questions about brain doping that were not possible to address in the past. For example, new testing procedures will need to be developed and we will be able to bring the entire NIH infrastructure to this task."

While “doping” is now accepted as a problem among athletes, it is less widely known that so-celled “brain doping” has been affecting the competitive balance in scientific research as well. It is for this reason that NIH is collaborating with the World Anti-Doping Authority (WADA), which has led the fight against doping in athletics, to create the World Anti Brain Doping Authority (WABDA). “Because brain doping is not just an American problem,” said Richard Pound, the current Director of WADA and acting Director of WABDA until a permanent head can be found, “we are working with the European Union’s research funding agency, the European Commission Research, to make sure WABDA is effective.

NABDAG will be established within the NIH Office of Intramural Research and administered by the National Institute of Mental Health (NIMH). Additional support for the center will come from the NIH Office of the Director, the National Institute on Drug Abuse (NIDA) and the Center for Scientific Review (CSR). The research activities of NABDAG will take place on the NIH Bethesda campus. An additional focus of NABDAG will be to provide training opportunities for students and established scientists from developing countries and from minority groups in the United States.

Together with WABDA, NABDAG will work to develop the international rules for the use of performance enhancing drugs among scientists as well as testing and punishment procedures. Most importantly they will administer the World Anti Brain-Doping Code, a set of uniform anti-brain doping rules. The NIH and European Commission have formally adopted this Code for the conduct of all scientists which receive funding in any form (intramural or extramural) from these agencies. The Code includes regulations on which drugs are prohibited, what the recommended testing procedures should be, and what the punishments should be for positive tests. More information on the WABDA Code can be found at http://wabda.org/. We note that the implementation will include testing of all NIH funded scientists both at the time they receive funding as well as at random times during the course of working on an NIH funded project. Testing will also be implemented at all NIH-funded or NIH-hosted events such as conferences and workshops and at grant review panels.

NIMH, NIDA, and CSR are among the 27 institutes and centers at the NIH, an agency of the Department of Health and Human Services. The NIMH mission is to reduce the burden of mental and behavioral disorders through research on mind, brain, and behavior. More information is available at the NIMH website http://www.nimh.nih.gov. The National Institute on Drug Abuse is a component of the National Institutes of Health, U.S. Department of Health and Human Services. NIDA supports most of the world's research on the health aspects of drug abuse and addiction. The Institute carries out a large variety of programs to ensure the rapid dissemination of research information to inform policy and improve practice. Fact sheets on the health effects of drugs of abuse and further information on NIDA research can be found on the NIDA web site at http://www.drugabuse.gov. The Center for Scientific Review organizes the peer review groups that evaluate the majority of grant applications submitted to the National Institutes of Health. CSR recruits about 18,000 outside scientific experts each year for its review groups. CSR also receives all NIH and many Public Health Service grant applications — about 80,000 a year — and assigns them to the appropriate NIH Institutes and Centers and PHS agencies. CSR’s primary goal is to see that NIH applications receive fair, independent, expert, and timely reviews that are free from inappropriate influences so NIH can fund the most promising research. For more information, visit http://www.csr.nih.gov.

The National Institutes of Health (NIH) — The Nation's Medical Research Agency — includes 27 Institutes and Centers and is a component of the U.S. Department of Health and Human Services. It is the primary federal agency for conducting and supporting basic, clinical and translational medical research, and it investigates the causes, treatments, and cures for both common and rare diseases. For more information about NIH and its programs, visit www.nih.gov.

Saturday, March 29, 2008

Metagenomics Education

Just a quick one here. I was reminded recently about an interesting publication about metagenomics education in which some people might be interested. It is by Anne Jurkowski, Ann Reid and Jay Lebov and was published in a journal called CBE Life Sciences Education. This journal, though not fully Open Access is freely available online.

I think the article is a useful callto arms for educators to get ahead of the curve and to start thinking about ways to teach metagenomics BEFORE it becomes an old field (i.e, while it is hot, and who knows how long that will last).

Wednesday, March 26, 2008

A different kind of Open Science - the need to track funding sources and conflicts of interest

Well, the News is abuzz with discussion of a controversy involving lung cancer studies that were funded by tobacco associated money (e.g., see MSNBC and TIME and the NY Times). The issue is that apparently the source of the money was hidden through some sort of laundering of the money through a foundation.

As many readers know, I am a bit obsessed with open access to scientific research publications. This here is a case where the need for openness goes well beyond publications. Here there is a need for openness about funding and conflicts of interest and the roles of all participants. In this case, I am not sure what could have been done by the journals involved to vet the funding of the project more carefully. But nevertheless, science in general can be severely hurt whenever there are cases of even the appearance of conflict of interest. I do believe that open access journals help in this in that anyone, anywhere, can look at the publication as well as the descriptions of the funding sources and the authors contributions. The more eyes we have on research products, the more likely problems will be discovered and (possibly) the less likely it will be to happen again.

Friday, March 21, 2008

Happy to get this email on NIH Policy on Enhancing Public Access to Archived Publications ....

Well, the new NIH policy is not perfect, but it is a good first step. And I must say, I got a tingle of excitement when I got this email from the UC Davis Administration:
March 20, 2008

I am writing to alert you to a new requirement for investigators who receive funding from the NIH. Under the Consolidated Appropriation Act of 2008, the NIH will require that all NIH-funded investigators submit or arrange for the submission of an electronic version of their final, peer-reviewed manuscripts accepted for publication to the National Library of Medicine’s PubMed Central. PubMed Central (PMC) is the NIH’s publicly-accessible, digital archive of full-text, peer-reviewed journal articles. This new law requires manuscripts to be made publicly available via PMC no later than 12 months after the official date of publication. Please note:

1. This requirement applies to all peer-reviewed articles that arise, in whole or in part, from direct costs funded by NIH, or from work of NIH staff, that are accepted for publication on or after April 7, 2008.

2. UC Davis investigators are responsible for ensuring that any publishing or copyright agreements concerning submitted articles fully comply with this requirement. To satisfy this obligation, UC Davis investigators should enclose a copy of the one-page letter prepared by the UC Office of the President with any articles submitted to publishers for possible publication either at the time the article is submitted or with the publication agreement. The subject letter is available at: http://www.ucop.edu/raohome/cgmemos/08-05a.pdf).

3. The final, peer-reviewed manuscript submitted to PMC must include all graphics and supplemental material that are associated with the article.

4. Beginning May 25, 2008, anyone submitting an application, proposal or progress report to the NIH is also required to include the PMC reference number when citing applicable articles arising from NIH-funded research. This requirement applies to proposals submitted to the NIH for the May 25, 2008 due date, as well as subsequent due dates.

5. Certain publishers have agreed to automatically submit articles to PMC on behalf of the authors. The list of these publishers can be accessed at http://publicaccess.nih.gov/submit_process_journals.htm

Even if a publisher is on this list, UC Davis investigators should verify that their published articles will be submitted to PMC in compliance with the law. If the publisher is not on the list, UC Davis investigators are responsible for submitting their articles to PMC and verifying receipt of the article.

6. Failure to comply with this policy may jeopardize future NIH funding.

Additional information about this law is available at http://publicaccess.nih.gov. Also, the UC Davis Library can provide further assistance to investigators. Please see http://www.lib.ucdavis.edu/dept/hsl/resources/other/nihmandate/ or contact the Library’s sciences department heads:

Breaking News: PZ Myers expelled from Expelled (the Movie)

Everyone must read, immediately, Pharyngula now.

PZ Myers writes about how he was expelled from the movie Expelled (a pro Intelligent Design movie). And the best part - Richard Dawkins was allowed in ...

Monday, March 17, 2008

Genomes of Energy and the Environment - JGI Users Meeting

Just a little post here --- if you are interested in Metagenomics, or Bioenergy or Microbes, consider going to the JGI Users Meeting March 26-28. Speakers include at least one Nobel Laureate (Steve Chu) as well as Mitch Sogin, Jill Banfield, Terry Hazen, and many others.

Friday, March 14, 2008

Eisen Resigns in Disgrace Over Scandal #FSN #PLoSTitution

By Saul Jacobson and Frank Tepedino, Asociated Press Writers

(03-13-2008) 19:50 PDT San Francisco (AP) --

In a startlingly swift fall from grace, the new Academic Editor in Chief of PLoS Biology Jonathan Eisen resigned Wednesday after getting caught in a pay-for-access scandal that made a mockery of his straight-arrow “open access only” image and left him facing the prospect of criminal charges and perhaps permanent exclusion from journal editorial boards.

"I cannot allow my private failings to disrupt the people's work," Eisen said, his weary-looking brother and Public Library of Science (PLoS) founder, Michael, standing at his side, again, as the closed access-fighting scientist once known as Mr. Open Access answered for his actions for the second time in three days.

He made the announcement without securing a plea bargain with NIH prosecutors, though an NIH official said the former PLoS Academic Editor in Chief was still believed to be negotiating one. The official spoke on condition of anonymity because of the sensitivity of the case.

Eisen will be succeeded on Monday by Alex Gann, a fellow scientist who becomes PLoS Biology’s first foreign-born Academic Editor in Chief and the nation's first legally blind chief editor.

The resignation brought the curtain down on a riveting three-day drama — played out, sometimes, as farce — that made Eisen an instant punchline on science blogs and fascinated Americans with the spectacle of a crusading scientist exposed as a hypocrite.

His dizzying downfall was met with glee and the popping of champagne corks among many on Crinan Street, where Eisen was seen as a sanctimonious bully for attacking high prices and abusive access practices in the publishing industry when he was a rising Academic Editor at PLoS Biology. And his resignation brought relief at PLoS headquarters in San Francisco after days of excruciating tension and uncertainty.

"Some rules can't be broken, and when they are broken there are consequences," said Harold Varmus, an Open Access advocate and ex-head of the NIH. "In this case, one of the most promising careers I've seen in a generation."

The scandal erupted Monday after NIH officials disclosed that a wiretap had caught the 39-year-old father of two spending thousands of grant dollars on journal articles about evolution at a fancy Washington hotel on the night before Darwin Day.

Investigators said he had arranged for a journal editor named Kristen to take the train down from New York while he was in the nation's capital to testify before a congressional subcommittee about the publishing industry.

Late Wednesday, the New York Times reported that her real name is Emma Hill. She declined to comment when asked by the Times when she first met Eisen and how many times she had helped him purchase and download closed access journal articles.

It was unclear whether she would face charges; attorney David Bora confirmed that he represents the same woman in the Times story but wouldn't comment further.

With every development, it became increasingly clear that Eisen, politically, was finished.

NIH enforcement officials said the Editor in Chief — the scientific heir to the PLoS banner — had spent multiple entire evenings downloading articles and had spent tens of thousands of grant dollars, and perhaps as much as $80,000, on high-priced Nature articles which cost as much as $35 each.

Senior Eisen aides, speaking on condition of anonymity because of the sensitivity of the matter, said Eisen had been informed Friday by NIH prosecutors that he was linked to the grant money laundering ring.

They said he had kept it to himself through Saturday night, when he attended the annual dinner of the National Academy of Sciences in Washington. That night a reporter kept calling cell phones of Eisen aides.

Eisen first shared the news Sunday with his brother at his house in Davis, and after several excruciating hours they told their family, the aides said. By Sunday evening Eisen had called top advisers, personal friends and PLoS loyalists. The little band huddled in the house until midnight.

After making a watery-eyed, non-specific public apology Monday with his brother by his side, Eisen continued to talk to family and advisers through Tuesday. By Wednesday morning, aides said, he had decided to resign.

He and his brother rode in a black SUV from the Davis house to PLoS headquarters in San Francisco to announce his resignation — a trip whose every move was captured by TV helicopters. During the news conference, he and his brother stood inches apart, never touching as they entered or left the room.

Speaking in a strong and steady voice, he apologized for his actions and said: "Over the course of my public life, I've insisted, I believe correctly, that scientists regardless of their position or power take responsibility for their conduct. I can and will ask no less of myself."

He did not address the allegations in any detail in the less than three-minute statement, and left without taking questions.

Officials said that Gann asked for the Monday hand-over because he needed more time to prepare and wanted Eisen to say the proper goodbye to his staff.

In a statement issued after Eisen quit, NIH Attorney Lisa Coffmancini, the chief grant money abuse prosecutor in California, said: "There is no agreement between this office and Eisen relating to his resignation or any other matter."

Among the possible charges that law enforcement authorities said could be brought against the former editor in chief: soliciting and paying for journal access; violating the Consolidated Appropriations Act, the 2008 federal law that makes it a crime to publish NIH funded research in non Open Access journals; and illegally arranging cash transactions to conceal their purpose.

Eisen, a graduate of Harvard University and Stanford graduate school, could also be disdoctorated. In California, a scientist can lose his license to practice for failing to "conduct himself both professionally and personally, in conformity with the standards of conduct imposed upon members of the scientific community."

It was a spectacular collapse for a man who cultivated an image as a hard-nosed scientist hell-bent on cleansing the state of corruption in scientific publishing. He served four terms as an Academic Editor at PLoS Biology, earning the nickname "Sheriff of Open Access," and was elected Academic Editor in Chief with a record share of the vote in 2008. The tall, athletic, square-jawed Eisen was sometimes mentioned as a potential candidate for president of the American Academy of Publishers.

But he also made powerful enemies, many of whom complained that he was abusive and self-righteous.

"I really don't feel vindicated," said Philip Campbell, the Editor in Chief of Nature who lost many papers to PLoS Biology via Eisen’s efforts. But he added: "One of the many things I said was that Jonathan Eisen had one set of rules for himself and one set for everyone else. I never would have imagined it could be so glaring."

Publishers on the floor of American Academy of Publisher’s annual meeting were transfixed by TV monitors broadcasting Eisen’s resignation, and his ruin drew scattered applause from publishers as they went about buying and selling articles. One said some firms even cracked champagne open — a ritual usually reserved for when subscription fees hit a milestone.

Gann said in a statement that he was saddened, but added: "It is now time for PLoS to get back to work as the people and scientist expect from us."

Barely known outside of his Cold Spring Harbor political base, Alex Gann, 53, has been in publishing since his election to the Nature editorial board in 1985.

Though legally blind, he has enough sight in his right eye to walk unaided, recognize people at conversational distance and even read if the text is placed close to his face.

While Eisen was famously abrasive, uncompromising and even insulting, Gann has built a reputation as a conciliator, and lawmakers quickly embraced the new order.

"The first thing he can and I think he will do is end the era of accusation and contempt and ridicule," said PLoS Co-Founder Pat Brown. "I think everyone will be better off because of it."






------------------------------------------------------------------
With apologies to those mentioned above and thanks to the real Associated Press story about Governor Spitzer by Verena Dobnik and Michael Gormley

Sunday, March 09, 2008

Wall Street Journal on Open Access

There is a nice essay (Information Liberation) by Daniel Akst in the Wall Street Journal on Open Access to scientific publications (thanks to Bora for pointing this out). He leads of the essay saying
If your child has a life-threatening disease and you're desperate to read the latest research, you'll be dismayed to learn that you can't -- at least not without hugely expensive subscriptions to a bevy of specialized journals or access to a major research library.

Your dismay might turn to anger when you realize that you paid for this research.
Go to the WSJ site to read the rest but he has some useful points about OA including:
  • That academic societies and their journals should be able to survive even if they provide the publications for free
  • That "barriers to the spread of information are bad for capitalism"
  • That open access can help limit plagiarism (by allowing anyone to search for copied text as has been done recently for a variety of literary works)
  • That open access to information can help speed development in impoverished nations
  • And his final point: "The challenge, in the coming new world of open access, will be keeping the best of the current system while jettisoning the rest. Maybe some scholar would like to study the question -- and publish his findings for all to see."

Friday, March 07, 2008

Open Evolution

I am starting a new blog theme here on Open Evolution. By that I mean, evolutionary biology studies that are in some form of open science format. This would include Open Access evolution publications, open source evolution programs, open data on evolution projects, etc.

Today I am focusing on Open Access publications with interesting Evolution themes.

First, there are some fully Open Access journals with a specific focus on Evolution (I found some of these through the Lund Univeristy's Directory of Open Access Journals). These include
There are of course other fully OA journals that have a decent chunk of the papers on some evolution related topic:
If anyone knows of any other Open Access evolution journals, please let me know.

Monday, March 03, 2008

Genomics Blogger Dissed by the New York Times

Well, the New York Times has an article today on Knome, a company that is charging people $350,000 to have their genome sequenced. They have two people signed up so far. Amy Harmon, the author of the Times article, interviewed me by email for her article, mostly because I had blogged extensively about the recent AGBT meeting where many of these sequencing companies had presented their latest goodies. But alas, being a genomics blogger apparently does not carry as much weight as being (1) Jim Watson (2) someone who pays 350,000 to have their genome sequenced (3) running a genome center (Richard Gibbs) or (4) starting Knome (George Church). And so my quotes got left on the proverbial cutting room floor. Go figure.

Too bad for the Times, as I think I my quotes were pretty good. Although in all honesty, they would not have gone too well with the final article, which has some nice cohesion to it.

I should note that, other than the lack of my quotes, one thing that got left out of the Times article is any discussion of the quality of the genome sequence that will be provided by Knome. One would hope that 350K would buy a high quality genome sequence but it is unclear how good it will be. I note they claim they will provide analysis of the genome too, but as with other companies that cater to the rich and famous, details are limited on their web site.

This reminds me of a funny scene I witnessed involving Craig Venter and a wealthy friend of his. This was the day before Craig's personal genome was set to come out in PLoS Biology and the friend was asking Craig what it cost to sequence his genome. Craig said something to the effect of "man millions of dollars" and then Craig said, "but now it would cost only about 300K." The friend was intrigued. And Craig asked if he was interested and the friend, without seeming in any way to be joking, said, "sure sign me up." I guess, when money is abundant, why not get your genome sequenced? Maybe I should have told Harmon this story --- then I might have gotten in the Times.

Sunday, March 02, 2008

Cool new tool to help decide where to submit a scientific paper and what scientists are working on

Just found out about a freaky and cool new tool called Jane - the Journal/Author Name Estimator. For more on the approach see this paper in Bioinformatics.

Here is how it works -- you paste some text into a box and you can get the tool to suggest a journal that is most suitable for your work. Or you can have the tool search for an author who is doing work similar to what is in the text (this is useful to find reviewers or papers to look at)

One thing I like about the journal tool is that it highlights which journals are Open Access and which are deposited in Pubmed Central. I have tried it out in a few different ways and it seems pretty useful. So if you want guidance on where to submit a paper or who might make a good reviewer of a paper, check it out. It seems like it would make a good tool to help reporters find someone to interview too.

Friday, February 29, 2008

The iPlant Collaborative - a new (and seemingly cool) collaborative informatics project

Just found out (from Steve Rounsley) about what seems to be a cool collaborative computational project on plants - the Iplant Collaborative. From their site:
The iPlant Collaborative (iPC) is a distributed, cyberinfrastructure-centered, international community of plant and computing researchers enabling new conceptual advances through computational thinking, and addressing an evolving array of the most compelling grand challenges in the plant sciences and associated, cutting-edge research challenges in the computing sciences.
For more info got to their web site. Also - they are having their 1st conference which apparently will be webcasted. So apparently anyone can watch.

Thursday, February 28, 2008

iSEEM Wants You --- We are Recruiting Post Docs in Metagenomics Informatics

I am pleased to announce that a new project on "Integrating Statistical Evolutionary, and Ecological Approaches to Metagenomics (iSEEM)" is getting up and running. The iSEEM Project, funded by the Gordon and Betty Moore Foundation, takes an integrated, interdisciplinary approach to metagenomic analysis.

The project spans three labs (mine and those of
Katherine Pollard (who is in the Davis Genome Center where I am) and Jessica Green (who is at U. Oregon)), each with different areas of focus. Overall, the plan is to develop and apply novel methods for analyzing metagenomic data with a focus on three main topics: phylogenetic characterization of organisms, ecological diversity, and population genomics. We will be posting more detail about the project at http://iseem.org.

We are seeking five post-doctoral scientists and a bioinformatics engineer to work on methodology for analysis of metagenomic data as part of this collaborative project. Each position will be associated with one of the PIs at their home institution. If you are interested in microbial diversity, metagenomics, or genome evolution, and are looking for a post doc and want to be part of a interdisciplinary collaborative project, please apply.

More detail on the jobs is below:

Qualifications
  • We are looking for people with a demonstrated interest in working at the interface between the quantitative and biological sciences. We will offer a generous salary and benefits commensurate with experience.
  • Postdocs: Applicants should have a PhD in a biological, computational, mathematical, or statistical field. Programming skills are highly desirable.
  • Bioinformatics Engineer: Applicants should have substantial experience with database programming (e.g. SQL), scripting (e.g. Perl or Python), and bioinformatics tools.

Term: Appointments will last 2 years beginning in Summer 2008.

To Apply: Please apply using our online application system. You will be asked for:
  1. a brief cover letter explaining your background, career interests, and preferred geographical location for work (if any),
  2. CV (including publications),
  3. names and contact information for three references.
Online Application System:

Wednesday, February 27, 2008

Punctuated equilibrium of my blog ... and a look at an old science rap

Well, with my new role in PLoS Biology as Academic Editor in Chief, I had planned to start blogging more about PLoS and PLoS Biology. And I will. However, I am going to have to do a half effort on this for at least a week or two as I have a lovely ailment called trigger finger which is making typing rather awkward. So in lieu of a detailed blog I simply have to make a brief comment on one of the articles in this months issue

The article in which I am interested is a nice primer on mutational meltdown in mammalian mitochondrial genomes by Dave Rand. This is in relation to a paper by Stewart et al. in this issue. But enough about science. For those who do not know, Dave Rand published what I believe was the first rap in a scientific article. In an article in Genetics he presented a rap about repeat induced point mutation (something known as RIPPING). This RAP about RIPPING I think was presented at the Evolution meeting in Berkeley (I think I was at the talk where he did the RAP but I am not sure --- I could have, like Andy Pettite, misremembered the whole thing).

And well, here it is:
Yo! you’ve got my DNA and you think it is a-RIPping,
Your dog is so excited her saliva is a-dripping,
She seems to think the polymerase is doing some a-skipping,
But then again, you never know, she might just be a-quipping.
Are you sure induction by repeats did make it happen?
If it’s just associated, why not call it RAPpin’?
What motifs are necessary to keep the strands a-snappin’?
I don’t know and I don’t care, but something did some zappin’.

Tuesday, February 26, 2008

A really good day at work

Overall, I like being a scientist. But some of the days can be quite dreary. As a grad. student you slog through days that resemble the reliving of events in Groundhog Day. Except that they are actually different days - they just seem the same. As a post doc you struggle to get work done to then get a job (OK, I skipped a post doc, but I heard about what it is like from others). And as a faculty member you spend an absurd amount of time wasted on useless administrative activities. And I sometimes seem to be stuck in these days a lot.

But today was a really good day. It was my first day after it was announced that I was the new Academic Editor in Chief of PLoS Biology. And I got lots of positive feedback from friends, colleagues and even strangers. And then we had "lab meeting" which I decided to turn into a picnic/hike. We went to a little spot only 2 miles from the UC Davis Genome Center, that was as close to wilderness as you can find in Davis. And I think everyone had fun, getting away from the grind AND I brought my 3 year old daughter too.

And this reminds me to say - in the past I have avoided talking about people in my lab in my blog because it seemed like it would be nice to give them some space from my blabbing. But today I was reminded of just how much I owe them. They of course do all the work that I end up getting credit for. So today I thank the current lab members specifically (Jenna Morgan, Amber Hartman, Marcel Huntemann, Dongying Wu, Martin Wu, and Sourav Chatterji) and with their permission you will probably be hearing more about them later.

Monday, February 25, 2008

PLoS Biology 2.0

PLoS Biology - www.plosbiology.org

Well, I guess it is official now so I should post about it here. As for this afternoon, I am now the new Academic Editor in Chief of PLoS Biology. To read more about this new role of mine go to the source. I will probably cross post the editorial here at some later point.
Also see other blogs/notes about this
For more on what I think about Open Access publishing see some of my other blogs on the issue.

Join the Public Library of Science

See Evolgen for a field guide to seminar audiences

EVOLGEN has a great post on the people at your department seminar. It is worth checking out and bringing with you next time you go to a seminar. Even better than looking around --- choose which one fits you the best. Up until a few years ago, I was definitely the pre-schooler
The pre-schooler: This dude uses the departmental seminar as his nap-time. He sits in the back, and when the lights go out, he's nodding off faster than River Phoenix in My Own Private Idaho. Someone get this guy a good-night's sleep.
I simply could not stay awake in any seminar so I would have to sit at the back. Now that I have kids, I am used to sleep deprivation and only rarely nod off in a seminar. I am no longer sure which one I am ...

Calling Michael Ashburner - please start a blog

I just got done with reading Won for All by Michael Ashburner which came out a few years ago. This book discussed the sequencing of the Drosophila genome by Celera and is a fascinating read. The best part is the snarky, obsessive, and funny commentary by Ashburner on the players and the games they played in the course of this project. I know the book came out a while ago, but if there ever was a science author perfectly built for blogging it has to be Ashburner. So I am calling all science blog fans to try and find a way to get him to start a blog. Also - he is a big supporter of open access publishing ... as can be seen in the videos below:






See also some other Reviews of the book:

Friday, February 22, 2008

How human rights work and microbial ecology are similar

The New York Times has a brief one page article in the Sunday Magazine on "The Forensic Humanitarian" In the article, Jim Giles describes the work of Patrick Ball, a statistician working on searching for evidence for war crimes. The work is clearly important. But what caught my attention was his method. Basically, he uses a form of mark-release-recapture statistics taken from ecology studies. In the article, Giles writes
To understand Ball’s accomplishments, you might start with the problem of counting rattlesnakes.
And then he goes on to describe how one can estimate how many rattlesnakes are in a population by marking ones that you find and then counting how many times you refind one you have marked previously. This is a method generally known as mark-release-recapture (e.g., see the Wikipedia entry here, which seems OK but I have not read too carefully).

Apparently, Ball uses a similar type of statistic to estimate the number of murders and/or deaths in particular areas, by looking for overlap among different reports of deaths.

What you say does this have to do with microbial ecology? Well, everything. Because one of the most common methods for estimating the number of microbial species in a sample is to use a gene survey method where one isolates DNA from environmental samples and then one looks in the DNA for multiple versions of a gene found in all species (the gene most commonly used is known as small subunit rRNA). The gene survey method is needed because appearance is not a robust method of identifying microbial species.

From the gene sampling data, one then compares each version of the gene to the others and counts how many times one sees the same form of the gene (suggesting that one has found two different cells of the same species). If one keeps seeing the same forms of the gene even with only a few samples, one would estimate there are few species in the sample. If one keeps seeing different forms of the gene, one would estimate there are many species in the sample.

Things that seem to be hidden - be the murders or populations of rattlesnakes or microbes - can still be studied with what the Times perfectly refers to as
"A statistical sleight of hand"

Forget McCain - the Real Problem in the New York Times - Adaptationism

Unlike McCain and his followers, generally, I really like the New York Times, especially the Science articles. But they do continuously irk me in one area - they repeatedly include lame adaptationistic evolutionary arguments. Now I am not the only person in the bloggersphere railing against people who say something MUST be adaptive simply because it is there (Larry Moran is the most persistent and interesting critic of adaptationism out there).

This week the Times has a doozy in the article on "Play" from the Sunday Magazine. In the article, Robin Henig writes
"If play is an extravagance, why has it persisted? It must have some adaptive function, or at least a benefit that outweighs its cost, or it would have been winnowed out by the forces of natural selection."
This is nearly a PERFECT adaptationistic line. And more importantly, it is simply not true. Some things persist in biological systems even when they have a cost that outweighs the benefit. And other things persist when they are neutral. Now I am not saying one way or another whether play has a benefit (the article is interesting and reasonably sound in many ways). But such statements as the one quoted above show a common misunderstanding of evolution. Evolution is NOT only about beneficial things persisting and detrimental ones going away. It is much more complex and interesting in fact. If you want to learn more about the perils of adaptationism, go to Larry Moran's blog. He really has some good stuff on it. Or go to the great gurus themselves - Gould and Lewontin.

Wednesday, February 13, 2008

Harvard's Moving To Open Access - Let's Use this to Push for OA at other places

Well, Harvard is frequently criticized for being a bit conservtive in responding to new ideas and initiatives. But it seems that recently Harvard is more like a oceangoing yacht than an oil tanker. And yesterday, the New York Times reported on a proposed new initiative that could make Harvard a leader in the movement towards "Open Access" publishing.

The Times reports
"Faculty members are scheduled to vote on a measure that would permit Harvard to distribute their scholarship online, instead of signing exclusive agreements with scholarly journals that often have tiny readerships and high subscription costs."
and
"Under the proposal Harvard would deposit finished papers in an open-access repository run by the library that would instantly make them available on the Internet. Authors would still retain their copyright and could publish anywhere they pleased — including at a high-priced journal, if the journal would have them."
In my opinion, there is no doubt this is a smart move. Sure, there are some potential downsides to open access. Some journals do good things and they may have to reinvent themselves to continue to bring in revenue. But welcome to the 21st century. It is not like other industries - like music and TV and movies and electronics and so on - have not had to reinvent themselves.

And the result are in --- Harvard approved the initiative (see here for example). Now - I think we should use this as an example to get other institutions to do the same thing. As reported in the Boston Globe, Harry Lewis a CS Professor at Harvard said:
"Harvard is in a unique position to do the right thing in the academic world," he said. "In this case, I think others will be emboldened by Harvard to follow its lead, and the course of collective action will be greater than the course any individual school will take."
I will do my best to get UC Davis to do the same thing, but given the animosity towards open access exhibited by our acting provost Barbara Horwitz, it may be a tough ride here. Fortunately, they are interviewing candidates for provost now and hopefully whomever they pick will be more supportive.

So - here is a call to others out there. Push for the same type of thing at your institution. I will be posting more on this in the coming days/weeks. Maybe collectively we can follow Harvard's lead on this and make Universities more about what they are supposed to be about - spreading knowledge.

Monday, February 11, 2008

Creating Mitochondria and a sign that we need open peer review

Well, since everyone else is posting about this I figured I should too (see for example Steven Salzberg's Blog, The Harvard Crimson, Pharyngula). If you have not heard yet, there is an article in the journal Proteomics that discussed how mitochondria must have been created by an intelligent designer.

For example on p8 the authors say:
"Alternatively, instead of sinking in a swamp of endless
debates about the evolution of mitochondria, it is better to
come up with a unified assumption that all living cells
undergo a certain degree of convergence or divergence to or
from each other to meet their survival in specific habitats.
Proteomics data greatly assist this realistic assumption that
connects all kinds of life. More logically, the points that show
proteomics overlapping between different forms of life are
more likely to be interpreted as a reflection of a single common
fingerprint initiated by a mighty creator than relying on
a single cell that is, in a doubtful way, surprisingly originating
all other kinds of life."
Say what you want about the journal Proteomics but boy did they screw this one up. I think they probably should have caught this without much effort but who knows exactly what happened. In all fairness to them, it is possible for weird thin gs to slip through at any journal. Reviewers are busy. Editors are busy. Everyone is busy. How can we prevent this from happening again. There is a simple change we could make that would help. It is called Open Peer review. That is, if reviewers names were publicly attached to papers they reviewed, and their reviews were published, we would be less likely to see things like this happen. Then, if someone agrees to do paper review, they would be careful about it. Sure, we would probably have a harder time getting reviewers, but that would be better than publishing crap.

What are the risks with Open Peer review? Well, some people might feel afraid to criticize others especially people with power. Well, I find this sad. Scientists criticize our collaborators and friends ALL the time in private. Why not be public about it? Aren;t we supposed to be searching for the truth? If we are, shouldn't we be willing to give our opinions in public forums?

Saturday, February 09, 2008

Marco Island - Saving Some of the Best for Last

Well, the Marco Island AGBT meeting just wrapped up and I they definitely saved some of the best for the end. I do not have a ton of time but here are my favorites:

Stephan Schuster gave a funny, entertaining and interesting talk on mammoth mitochondrial genomics. He riddled the talk with funny stories and one liners about his efforts to sequence old DNA and to publish the results. He did a good job of showing why Roche-454 sequencing is quite ideally suited for studies of old DNA.

John Leamon from Raindance Technologies summarize some of their work on droplet based microfluidics. He showed videos of droplets moving through their system and showed how it sould be used for various digital PCR-like activities.

But it was the last talk of the whole meeting that really did blow my mind. It was from Steve Turner from Pacific Biosciences. He presented an overview of their sequencing technology as well as a tiny bit of data. Now, normally I am uninterested in marketing talks where little data is presented. But this talk was different. First, their technology clearly has enormous potential for revolutionizing the sequencing field. Basically, what they are doing is reading the activity of a DNA polymerase as it replicates a single DNA molecule and they do it in real time. He referred to this as using the DNA polymerase as a sequencing engine and then he took the crowd through the details of the technology and some of the modifications they have made to make it work better. I will try to post later with more detail on their methods.

No - they are not quite ready for prime time yet. But the potential is pretty absurd. I see two key major advantages of their method if it can be fine tuned to work well -- (1) it is screamingly fast - because they let the polymerase do all the work in essence (2) it can potentially get long reads -- right now he claimed they could do up to 1500 base pairs and theoretically it could go much higher. If they can get this to work with reads of 10,000 bases for example, this will completely reconfigure the field. No longer will one have to worry about the complexities of mapping short reads as with many of the current "new" methods. And the long reads, coupled with many molecules per run, plus the high speed, this technology is the first I have seen that has shown some results and that could really lead to the $1000 human genome. Again, not clear when/if they will be ready for release to the world so don't hold off buying one of the other systems that currently work (i.e., Illumina, Roche, ABI Solid) if you want to do "next gen" sequencing. But this company is one to keep an eye on.


NOTE - SEE ALSO

Marco Island sequencing frenzy - are we getting lost in all the data?

So - the Marco Island meeting could be summed up as a sequencing frenzy. Everyone and their mother presented something about how the next generation sequencers are revolutionizing their work. People are using these systems (well, people are using mostly Illumina and Roche sequencers and some are using ABI Solid) to do all sorts of new things - RNAi, gene expression, methylation, mutations, population genetics, comparative genomics, metagenomics, infectious disease, etc etc etc.

Certainly, much of this new work is truly revolutionary. Sequencing has gotten so cheap, and so easy, relative to even 3-4 years ago, that it can be used in all sorts of new ways. And new developments in sequencing seem poised to happen too, to make sequencing even cheaper and even better. Sequencing will get even better for a few reasons.

First, the current players in the market (Roche, Illumina, and possibly soon ABI Solid) are improving both their systems and their informatics such that they are getting more and more robust and easy to use and producing more data (Roche for example presented details on how they can extend their sequence reads to ~350 base pairs mostly by software and reagent changes). Lots of companies can say "We have some sequencing system about ready to be introduced" And lots of them are doing this at this meeting. But there is nothing like having sequencers in the hands of scientists to really test how well they work and to really help push the development of the technology.

Second, it does seem like some competitors for Illumina and Roche are coming. ABI presented multiple results from ABI Solid technology that makes it seem like these systems are ready for prime time. Whether other systems are ready for prime time is unclear. Helicos presented what could be seen as data. It was disappointingly minimal on detail but though I am rooting for them, it was far from convincing. But the discussions in the hallway seemed to suggest that Helicos is getting close. And there are 5+ other players itching to get into the market (some of which are apparently presenting later today). Some will fail. Some will succeed. And as long as their are a couple of good systems, the competition will push further development and reductions in costs. Thus everyone at the meeting I talked to said basically the same thing --- this is an exciting time in sequencing.

So - sequencing is getting better and cheaper. That certainly will be good in many ways. But there are some negative aspects to this frenzy. I see two in particular. The first, which was discussed extensively at the meeting, is that nobody is really prepared to deal with the sheer volume of data coming out of these new systems. Data storage, transfer and analysis will unquestionably be the rate limiting steps in turining the new sequence data into knowledge.

And this is the other negative aspect of the new frenzy. Right now there seems to be a mad rush to apply the new sequencing methods to everything under the sun. And the data piles up. And piles up. And the biology seems to have taken a back seat in some cases. Perhaps the bext example of this is exemplified by something Neil Hall pointed out yesterday to me. There has been almost no mention at this whole meeting of things related to function of genes. For example, I have not heard "gene ontology" once. I do not think I have even heard "annotation" once. Function and process have been replaced by terms like "systems biology" and "SNPs" and "networks" and "massively parallel." We have in a way regressed in terms of treating organisms (or communities) as a black box. Fine scale detail has been lost in a sea of data. In a way, we have all become born again geneticists. And I do not mean to disparage genetics. But I mean the part of genetics that treats organisms as a bit of a black box and focuses just on transmission of traits. We need to find a way to not get lost in all the data. I am not sure how to do that, but when we do, then the full potential of the new sequencing methods will be realized.

Friday, February 08, 2008

Coolest Thing at Marco Island - The Polonator


Without a doubt, the coolest thing at AGBT/Marco Island is the Polonator. This is a new sequencing system build by Denaher Motion based on the polony method from George Church's lab. Now I have no idea if this machine even works. And even if it works, I have no idea how useful it will be. But the idea is brilliant and appealing. They are trying to be an "Open Development" Massively High Throughput Sequencing system. By Open they mean, they will use open source software (and would love developers to help) and will use non proprietary reagents and supposedly try to make everything as cheap as possible. They will still make the main piece of equipment and I assume this is what they hope to make money off of.

I went to a showing in a room nearby the seminar room. There were many skeptics there. Most were concerned about the read lenght coming from the machine. But it should get better. And if the price is a lot less for reagents and the machine than anyone else's system --- this could be an important player in the market.





See also

Most recent post

A ton to be thankful for -- here is one part of that - all the acknowledgement sections from my scholarly papers

So - it is another Thanksgiving Day and in addition to thinking about family, and football, and Alice's Restaurant, I also think a lot a...