It is the little things. The little things that can sometimes eat at you. And here is one of my little pet peeves. At some point - not sure how recently - NCBI changed the default database for Blastn searching to the "human G+T" database. This Db contains human genomic and transcriptomic data.
Showing posts with label NCBI. Show all posts
Showing posts with label NCBI. Show all posts
Thursday, August 30, 2012
NCBI. Why? Why? Why is the default database for blasting "human G+T"
Tuesday, February 08, 2011
Though I generally love NCBI, the Sequence/Short Read Archive (SRA) seems to have issues; what do others think?
Well, here goes. Hope to not get people from NCBI too pissed off here. Overall, I think NCBI is invaluable: GenBank. PubMed. PubMed Central (PMC) (well, I have some complaints about that but let's not get into those here -- I still like it), BLAST (Basic Local Alignment Search Tool) and a plethora of other tools, databases and resources. Generally, money well spent.
However, one database from NCBI is driving me a bit wacky these days. This is the Sequence Read Archive (SRA). Known to some as the "Short Read Archive" this database is supposedly for storing "sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome Analyzer®, Life Technologies AB SOLiD System® , Helicos Biosciences Heliscope®;, Complete Genomics®, and Pacific Biosciences SMRT®."
It certainly seems to be used for that function. But alas, storing sequence is not the only need here. Recovering sequence and making use of it is really the key. And this is the area I have been having trouble with (especially related to environmental studies like rRNA PCR and metagenomics). Rather than go on about my particular issues here (and thus possibly biasing the discussion too much), I am wondering what others think of the SRA? Usability? Ease of deposition? Ease of extraction? Missing features? Things it does or does not do well? Do we need a new system for environmental projects?
Any and all comments welcome here or on twitter or on Friendfeed or wherever. See Friendfeed stream below:
Here are some comments so far from twitter
However, one database from NCBI is driving me a bit wacky these days. This is the Sequence Read Archive (SRA). Known to some as the "Short Read Archive" this database is supposedly for storing "sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome Analyzer®, Life Technologies AB SOLiD System® , Helicos Biosciences Heliscope®;, Complete Genomics®, and Pacific Biosciences SMRT®."
It certainly seems to be used for that function. But alas, storing sequence is not the only need here. Recovering sequence and making use of it is really the key. And this is the area I have been having trouble with (especially related to environmental studies like rRNA PCR and metagenomics). Rather than go on about my particular issues here (and thus possibly biasing the discussion too much), I am wondering what others think of the SRA? Usability? Ease of deposition? Ease of extraction? Missing features? Things it does or does not do well? Do we need a new system for environmental projects?
Any and all comments welcome here or on twitter or on Friendfeed or wherever. See Friendfeed stream below:
Here are some comments so far from twitter
- digitalbio Sandra Porter I agree. RT @phylogenomics: Though I generally love NCBI, the Sequence/Short Read Archive (SRA) seems to hav… (cont) http://deck.ly/~XM75A
- lswenson Luke Swenson @phylogenomics I was JUST trying to navigate the SRA! There's no help section to be found, and forget about depositing sequences!
- audyyy Davis-Richardson @phylogenomics I can never tell if my submission went through without emailing support. Also, no FASTQ support?
- cabbageRed Rich C .@phylogenomics I agree, the SRA doesn't seem to be the easiest repository to search with what I believe to be "typical" NGS queries
Subscribe to:
Posts (Atom)
Most recent post
A ton to be thankful for -- here is one part of that - all the acknowledgement sections from my scholarly papers
So - it is another Thanksgiving Day and in addition to thinking about family, and football, and Alice's Restaurant, I also think a lot a...
-
Wow. Just wow. And not in a good way. Just got an email invitation to a meeting. The meeting is " THE FIRST ANNUAL WINTER Q-BIO ...
-
I have a new friend in Google Scholar Updates I have written about the Updates system before and if you want more information please see...
-
See Isolation and sequence-based characterization of a koala symbiont: Lonepinella koalarum Paper based on PhD thesis work of Katie Dahlha...
