Here are examples of some information I have found:
- From Jo Handelsman's review on metagenomics in 2004: "One of the indicators that cultured microorganisms did not represent much of the microbial world was the oft-observed "great plate count anomaly" (135)—the discrepancy between the sizes of populations estimated by dilution plating and by microscopy. This discrepancy is particularly dramatic in some aquatic environments, in which plate counts and viable cells estimated by acridine orange staining can differ by four to six orders of magnitude (66), and in soil, in which 0.1 to 1% of bacteria are readily culturable on common media under standard conditions (138, 139)."
- From Phil Hugenholtz's review in 2002: "under aerobic conditions, at moderate temperatures. Easily isolated organisms are the ‘weeds’ of the microbial world and are estimated to constitute less than 1% of all microbial species (this figure was estimated by comparing plate counts with direct microscopic counts of microorganisms in environmental samples; it has been called the “great plate-count anomaly” ). " Reference 1 is Staley JT and Kanopka 1985.
Anyone know of anything like this?