tag:blogger.com,1999:blog-10781944.post5498655336837511174..comments2024-03-28T00:36:36.460-07:00Comments on The Tree of Life: Correcting for rRNA copy # in qPCR experimentsJonathan Eisenhttp://www.blogger.com/profile/07953790938128734305noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-10781944.post-72445088370149377912013-02-06T11:19:55.960-08:002013-02-06T11:19:55.960-08:00Thanks .. and agree that ploidy could be a big big...Thanks .. and agree that ploidy could be a big big big problem.Jonathan Eisenhttps://www.blogger.com/profile/07953790938128734305noreply@blogger.comtag:blogger.com,1999:blog-10781944.post-10907424562832666742013-02-06T10:49:32.739-08:002013-02-06T10:49:32.739-08:00I have seen these papers cited when correcting for...I have seen these papers cited when correcting for rRNA gene copy number:<br />Klappenbach JA, Saxman PR, Cole JR, Schmidt TM (2001) rrndb: the<br />Ribosomal RNA Operon Copy Number Database. Nucl. Acids Res., 29,<br />181-184.<br /><br />Lee ZM-P, Bussema C, Schmidt TM (2009) rrnDB: documenting the number<br />of rRNA and tRNA genes in bacteria and archaea. Nucleic Acids Res, 37,<br />D489-D493.<br /><br />However, I wonder if anyone has ever thought about how ploidy would affect 16S rDNA qPCR? For example:<br />http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0016392<br /><br />If some species typically have 20 genome copies per cell and others have 1 genome copy per cell, this would make conversion of gene copy numbers to cells pretty much impossible unless you measure the ploidy for every species in your system. And if you don't do the conversion, then what do gene copy numbers mean? <br />Billyhttps://www.blogger.com/profile/18424769392487556145noreply@blogger.com